Interferometry in the context of Differential interference contrast microscopy


Interferometry in the context of Differential interference contrast microscopy

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⭐ Core Definition: Interferometry

Interferometry is a technique which uses the interference of superimposed waves to extract information. Interferometry typically uses electromagnetic waves and is an important investigative technique in the fields of astronomy, fiber optics, engineering metrology, optical metrology, oceanography, seismology, spectroscopy (and its applications to chemistry), quantum mechanics, nuclear and particle physics, plasma physics, biomolecular interactions, surface profiling, microfluidics, mechanical stress/strain measurement, velocimetry, optometry, and making holograms.

Interferometers are devices that extract information from interference. They are widely used in science and industry for the measurement of microscopic displacements, refractive index changes and surface irregularities. In the case with most interferometers, light from a single source is split into two beams that travel in different optical paths, which are then combined again to produce interference; two incoherent sources can also be made to interfere under some circumstances. The resulting interference fringes give information about the difference in optical path lengths. In analytical science, interferometers are used to measure lengths and the shape of optical components with nanometer precision; they are the highest-precision length measuring instruments in existence. In Fourier transform spectroscopy they are used to analyze light containing features of absorption or emission associated with a substance or mixture. An astronomical interferometer consists of two or more separate telescopes that combine their signals, offering a resolution equivalent to that of a telescope of diameter equal to the largest separation between its individual elements.

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👉 Interferometry in the context of Differential interference contrast microscopy

Differential interference contrast (DIC) microscopy, also called Nomarski interference contrast (NIC) or Nomarski microscopy, is an optical microscopy technique used to enhance the contrast in unstained, transparent samples. DIC works on the principle of interferometry to gain information about the optical path length of the sample, to see otherwise invisible features. A relatively complex optical system produces an image with the object appearing black to white on a grey background. This image is similar to that obtained by phase-contrast microscopy, but without the bright diffraction halo. The technique was invented by Francis Hughes Smith. The "Smith DIK" was produced by Ernst Leitz Wetzlar in Germany and was difficult to manufacture. DIC was then developed further by Polish physicist Georges Nomarski in 1952.

DIC works by separating a polarized light source into two orthogonally polarized mutually coherent parts which are spatially displaced (sheared) at the sample plane, and recombined before observation. The interference of the two parts at recombination is sensitive to their optical path difference (i.e. the product of refractive index and geometric path length). Adding an adjustable offset phase determining the interference at zero optical path difference in the sample, the contrast is proportional to the path length gradient along the shear direction, giving the appearance of a three-dimensional physical relief corresponding to the variation of optical density of the sample, emphasising lines and edges though not providing a topographically accurate image.

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Interferometry in the context of Paranal Observatory

Paranal Observatory is an astronomical observatory operated by the European Southern Observatory (ESO). It is located in the Atacama Desert of Northern Chile on Cerro Paranal at 2,635 m (8,645 ft) altitude, 120 km (70 mi) south of Antofagasta. By total light-collecting area, it is the largest optical-infrared observatory in the Southern Hemisphere; worldwide, it is second to the Mauna Kea Observatory on Hawaii.

The Very Large Telescope (VLT), the largest telescope on Paranal, is composed of four separate 8.2 m (320 in) telescopes. In addition, the four main telescopes can be used simultaneously for extra light gathering capacity, and for interferometry. Four auxiliary telescopes of 1.8 m (71 in) each are also part of the VLTI to make it available when the main telescopes are being used for other projects. The site also houses two survey telescopes with wide fields of view, the 4.0 m (160 in) VISTA and the 2.6 m (100 in) VLT Survey Telescope for surveying large areas of the sky; and two arrays of small telescopes called NGTS and SPECULOOS which are dedicated to searching for exoplanets.

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Interferometry in the context of Astrometry

Astrometry is a branch of astronomy that involves precise measurements of the positions and movements of stars and other celestial bodies. It provides the kinematics and physical origin of the Solar System and this galaxy, the Milky Way.

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Interferometry in the context of Radio astronomy

Radio astronomy is a subfield of astronomy that studies celestial objects using radio waves. It started in 1933, when Karl Jansky at Bell Telephone Laboratories reported radiation coming from the Milky Way. Subsequent observations have identified a number of different sources of radio emission. These include stars and galaxies, as well as entirely new classes of objects, such as radio galaxies, quasars, pulsars, and masers. The discovery of the cosmic microwave background radiation, regarded as evidence for the Big Bang theory, was made through radio astronomy.

Radio astronomy is conducted using large radio antennas referred to as radio telescopes, that are either used alone, or with multiple linked telescopes utilizing the techniques of radio interferometry and aperture synthesis. The use of interferometry allows radio astronomy to achieve high angular resolution, as the resolving power of an interferometer is set by the distance between its components, rather than the size of its components.

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Interferometry in the context of Beam splitter

A beam splitter or beamsplitter is an optical device that splits a beam of light into a transmitted and a reflected beam. It is a crucial part of many optical experimental and measurement systems, such as interferometers, also finding widespread application in fibre optic telecommunications.

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Interferometry in the context of Michelson interferometer

The Michelson interferometer is a common configuration for optical interferometry and was invented by the American physicist Albert Abraham Michelson in 1887. Using a beam splitter, a light source is split into two arms. Each of those light beams is reflected back toward the beamsplitter which then combines their amplitudes using the superposition principle. The resulting interference pattern that is not directed back toward the source is typically directed to some type of photoelectric detector or camera. For different applications of the interferometer, the two light paths can be with different lengths or incorporate optical elements or even materials under test.

The Michelson interferometer is employed in many scientific experiments and became well known for its use by Michelson and Edward Morley in the famous Michelson–Morley experiment (1887) in a configuration which would have detected the Earth's motion through the supposed luminiferous aether that most physicists at the time believed was the medium in which light waves propagated. The null result of that experiment essentially disproved the existence of such an aether, leading eventually to the special theory of relativity and the revolution in physics at the beginning of the twentieth century. In 2015, another application of the Michelson interferometer, LIGO, made the first direct observation of gravitational waves. That observation confirmed an important prediction of general relativity, validating the theory's prediction of space-time distortion in the context of large scale cosmic events (known as strong field tests).

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Interferometry in the context of Velocimetry

Velocimetry is the measurement of the velocity of fluids. This is a task often taken for granted, and involves far more complex processes than one might expect. It is often used to solve fluid dynamics problems, study fluid networks, in industrial and process control applications, as well as in the creation of new kinds of fluid flow sensors. Methods of velocimetry include particle image velocimetry and particle tracking velocimetry, Molecular tagging velocimetry, laser-based interferometry, ultrasonic Doppler methods, Doppler sensors, and new signal processing methodologies.

In general, velocity measurements are made in the Lagrangian or Eulerian frames of reference (see Lagrangian and Eulerian coordinates). Lagrangian methods assign a velocity to a volume of fluid at a given time, whereas Eulerian methods assign a velocity to a volume of the measurement domain at a given time. A classic example of the distinction is particle tracking velocimetry, where the idea is to find the velocity of individual flow tracer particles (Lagrangian) and particle image velocimetry, where the objective is to find the average velocity within a sub-region of the field of view (Eulerian).

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Interferometry in the context of Hologram

Holography is a technique that allows a wavefront to be recorded and later reconstructed. It is best known as a method of generating three-dimensional images, and has a wide range of other uses, including data storage, microscopy, and interferometry. In principle, it is possible to make a hologram for any type of wave.

A hologram is a recording of an interference pattern that can reproduce a 3D light field using diffraction. In general usage, a hologram is a recording of any type of wavefront in the form of an interference pattern. It can be created by capturing light from a real scene, or it can be generated by a computer, in which case it is known as a computer-generated hologram, which can show virtual objects or scenes. Optical holography needs a laser light to record the light field. The reproduced light field can generate an image that has the depth and parallax of the original scene. A hologram is usually unintelligible when viewed under diffuse ambient light. When suitably lit, the interference pattern diffracts the light into an accurate reproduction of the original light field, and the objects that were in it exhibit visual depth cues such as parallax and perspective that change realistically with the different angles of viewing. That is, the view of the image from different angles shows the subject viewed from similar angles.

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Interferometry in the context of Astronomical interferometer

An astronomical interferometer or telescope array is a set of separate telescopes, mirror segments, or radio telescope antennas that work together as a single telescope to provide higher resolution images of astronomical objects such as stars, nebulas and galaxies by means of interferometry. The advantage of this technique is that it can theoretically produce images with the angular resolution of a huge telescope with an aperture equal to the separation, called baseline, between the component telescopes. The main drawback is that it does not collect as much light as the complete instrument's mirror. Thus it is mainly useful for fine resolution of more luminous astronomical objects, such as close binary stars. Another drawback is that the maximum angular size of a detectable emission source is limited by the minimum gap between detectors in the collector array.

Interferometry is most widely used in radio astronomy, in which signals from separate radio telescopes are combined. A mathematical signal processing technique called aperture synthesis is used to combine the separate signals to create high-resolution images. In Very Long Baseline Interferometry (VLBI) radio telescopes separated by thousands of kilometers are combined to form a radio interferometer with a resolution which would be given by a hypothetical single dish with an aperture thousands of kilometers in diameter. At the shorter wavelengths used in infrared astronomy and optical astronomy it is more difficult to combine the light from separate telescopes, because the light must be kept coherent within a fraction of a wavelength over long optical paths, requiring very precise optics. Practical infrared and optical astronomical interferometers have only recently been developed, and are at the cutting edge of astronomical research. At optical wavelengths, aperture synthesis allows the atmospheric seeing resolution limit to be overcome, allowing the angular resolution to reach the diffraction limit of the optics.

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Interferometry in the context of Very long baseline interferometry

Very-long-baseline interferometry (VLBI) is a type of astronomical interferometry used in radio astronomy. In VLBI a signal from an astronomical radio source, such as a quasar, is collected at multiple radio telescopes on Earth or in space. The distance between the radio telescopes is then calculated using the time difference between the arrivals of the radio signal at different telescopes. This allows observations of an object that are made simultaneously by many radio telescopes to be combined, emulating a telescope with a size equal to the maximum separation between the telescopes.

Data received at each antenna in the array include arrival times from a local atomic clock, such as a hydrogen maser. At a later time, the data are correlated with data from other antennas that recorded the same radio signal, to produce the resulting image. The resolution achievable using interferometry is proportional to the observing frequency. The VLBI technique enables the distance between telescopes to be much greater than that possible with conventional interferometry, which requires antennas to be physically connected by coaxial cable, waveguide, optical fiber, or other type of transmission line. The greater telescope separations are possible in VLBI due to the development of the closure phase imaging technique by Roger Jennison in the 1950s, allowing VLBI to produce images with superior resolution.

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Interferometry in the context of List of largest optical reflecting telescopes

This list of the largest optical reflecting telescopes with objective diameters of 3.0 metres (120 in) or greater is sorted by aperture, which is a measure of the light-gathering power and resolution of a reflecting telescope. The mirrors themselves can be larger than the aperture, and some telescopes may use aperture synthesis through interferometry. Telescopes designed to be used as optical astronomical interferometers such as the Keck I and II used together as the Keck Interferometer (up to 85 m) can reach higher resolutions, although at a narrower range of observations. When the two mirrors are on one mount, the combined mirror spacing of the Large Binocular Telescope (22.8 m) allows fuller use of the aperture synthesis.

Largest does not always equate to being the best telescopes, and overall light gathering power of the optical system can be a poor measure of a telescope's performance. Space-based telescopes, such as the Hubble Space Telescope, take advantage of being above the Earth's atmosphere to reach higher resolution and greater light gathering through longer exposure times. Location in the northern or southern hemisphere of the Earth can also limit what part of the sky can be observed, and climate conditions at the observatory site affect how often the telescope can be used each year.

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Interferometry in the context of Aperture synthesis

Aperture synthesis or synthesis imaging is a type of interferometry that mixes signals from a collection of telescopes to produce images having the same angular resolution as an instrument the size of the entire collection. At each separation and orientation, the lobe-pattern of the interferometer produces an output which is one component of the Fourier transform of the spatial distribution of the brightness of the observed object. The image (or "map") of the source is produced from these measurements. Astronomical interferometers are commonly used for high-resolution optical, infrared, submillimetre and radio astronomy observations. For example, the Event Horizon Telescope project derived the first image of a black hole using aperture synthesis.

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Interferometry in the context of LIGO

The Laser Interferometer Gravitational-Wave Observatory (LIGO) is a large-scale physics experiment and observatory designed to detect cosmic gravitational waves and to develop gravitational-wave observations as an astronomical tool. Prior to LIGO, all data about the universe has come in the form of light and other forms of electromagnetic radiation, from limited direct exploration on relatively nearby Solar System objects such as the Moon, Mars, Venus, Jupiter and their moons, asteroids etc, and from high energy cosmic particles. Initially, two large observatories were built in the United States with the aim of detecting gravitational waves by laser interferometry. Two additional, smaller gravitational wave observatories are now operational in Japan (KAGRA) and Italy (Virgo). The two LIGO observatories use mirrors spaced 4 km apart to measure changes in length—over an effective span of 1120 km—of less than one ten-thousandth the charge diameter of a proton.

The initial LIGO observatories were funded by the United States National Science Foundation (NSF). They were conceived, built, and are operated by Caltech and MIT. They collected data from 2002 to 2010, but no gravitational waves were detected during that period.

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Interferometry in the context of Electronic paper

Electronic paper or intelligent paper, is a display device that reflects ambient light, mimicking the appearance of ordinary ink on paper – unlike conventional flat-panel displays which need additional energy to emit their own light. This may make them more comfortable to read, and provide a wider viewing angle than most light-emitting displays. The contrast ratio in electronic displays available as of 2008 approaches newspaper, and newly developed displays are slightly better. An ideal e-paper display can be read in direct sunlight without the image appearing to fade.

Technologies include Gyricon, electrowetting, interferometry, and plasmonics.Many electronic paper technologies hold static text and images indefinitely without electricity. Flexible electronic paper uses plastic substrates and plastic electronics for the display backplane. Applications of e-paper include electronic shelf labels and digital signage, bus station time tables, electronic billboards, smartphone displays, and e-readers able to display digital versions of books and magazines.

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