Denitrification in the context of "Reactive nitrogen"

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⭐ Core Definition: Denitrification

Denitrification is a microbially facilitated process where nitrate (NO3) is reduced and ultimately produces molecular nitrogen (N2) through a series of intermediate gaseous nitrogen oxide products. Facultative anaerobic bacteria perform denitrification as a type of respiration that reduces oxidized forms of nitrogen in response to the oxidation of an electron donor such as organic matter. The preferred nitrogen electron acceptors in order of most to least thermodynamically favorable include nitrate (NO3), nitrite (NO2), nitric oxide (NO), nitrous oxide (N2O), finally resulting in the production of N2, completing the nitrogen cycle. Denitrifying microbes require a very low oxygen concentration of less than 10%, as well as organic C for energy. Since denitrification can remove NO3, reducing its leaching to groundwater, it can be strategically used to treat sewage or animal residues of high nitrogen content. Denitrification can leak N2O, which is an ozone-depleting substance and a greenhouse gas that can have a considerable influence on global warming.

The process is performed primarily by heterotrophic bacteria (such as Paracoccus denitrificans and various pseudomonads), although autotrophic denitrifiers have also been identified (e.g., Thiobacillus denitrificans). Denitrifiers are represented in all main phylogenetic groups. Generally, several species of bacteria are involved in the complete reduction of NO3 to N2, and more than one enzymatic pathway has been identified in the reduction process. The denitrification process does not only provide energy to the organism performing nitrate reduction to dinitrogen gas, but also some anaerobic ciliates can use denitrifying endosymbionts to gain energy similar to the use of mitochondria in oxygen respiring organisms.

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👉 Denitrification in the context of Reactive nitrogen

Reactive nitrogen ("Nr"), also known as fixed nitrogen, refers to all forms of nitrogen present in the environment except for molecular nitrogen (N
2
). While nitrogen is an essential element for life on Earth, molecular nitrogen is comparatively unreactive, and must be converted to other chemical forms via nitrogen fixation before it can be used for growth. Common Nr species include nitrogen oxides (NO
x
), ammonia (NH
3
), nitrous oxide (N
2
O
), as well as the anion nitrate (NO
3
).

Biologically, nitrogen is "fixed" mainly by the microbes (eg., Bacteria and Archaea) of the soil that fix N
2
into mainly NH
3
but also other species. Legumes, a type of plant in the Fabacae family, are symbionts to some of these microbes that fix N
2
. NH
3
is a building block to Amino acids and proteins amongst other things essential for life. However, just over half of all reactive nitrogen entering the biosphere is attributable to anthropogenic activity such as industrial fertilizer production. While reactive nitrogen is eventually converted back into molecular nitrogen via denitrification, an excess of reactive nitrogen can lead to problems such as eutrophication in marine ecosystems.

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In this Dossier

Denitrification in the context of Wildlife management

Wildlife management is the management process influencing interactions among and between wildlife, its habitats and people to achieve predefined impacts. Wildlife management can include wildlife conservation, population control, gamekeeping, wildlife contraceptive and pest control.

Wildlife management aims to halt the loss in the Earth's biodiversity, by taking into consideration ecological principles such as carrying capacity, disturbance and succession, and environmental conditions such as physical geography, pedology and hydrology. Most wildlife biologists are concerned with the conservation and improvement of habitats; although rewilding is increasingly being undertaken. Techniques can include reforestation, pest control, nitrification and denitrification, irrigation, coppicing and hedge laying.

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Denitrification in the context of Nitrogen cycle

The nitrogen cycle is the biogeochemical cycle by which nitrogen is converted into multiple chemical forms as it circulates among atmospheric, terrestrial, and marine ecosystems. The conversion of nitrogen can be carried out through both biological and physical processes. Important processes in the nitrogen cycle include fixation, ammonification, nitrification, and denitrification. The majority of Earth's atmosphere (78%) is atmospheric nitrogen, making it the largest source of nitrogen. However, atmospheric nitrogen has limited availability for biological use, leading to a scarcity of usable nitrogen in many types of ecosystems.

The nitrogen cycle is of particular interest to ecologists because nitrogen availability can affect the rate of key ecosystem processes, including primary production and decomposition. Human activities such as fossil fuel combustion, use of artificial nitrogen fertilizers, and release of nitrogen in wastewater have dramatically altered the global nitrogen cycle. Human modification of the global nitrogen cycle can negatively affect the natural environment system and also human health.

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Denitrification in the context of Biogeochemical cycle

A biogeochemical cycle, or more generally a cycle of matter, is the movement and transformation of chemical elements and compounds between living organisms, the atmosphere, and the Earth's crust. Major biogeochemical cycles include the carbon cycle, the nitrogen cycle and the water cycle. In each cycle, the chemical element or molecule is transformed and cycled by living organisms and through various geological forms and reservoirs, including the atmosphere, the soil and the oceans. It can be thought of as the pathway by which a chemical substance cycles (is turned over or moves through) the biotic compartment and the abiotic compartments of Earth. The biotic compartment is the biosphere and the abiotic compartments are the atmosphere, lithosphere and hydrosphere.

For example, in the carbon cycle, atmospheric carbon dioxide is absorbed by plants through photosynthesis, which converts it into organic compounds that are used by organisms for energy and growth. Carbon is then released back into the atmosphere through respiration and decomposition. Additionally, carbon is stored in fossil fuels and is released into the atmosphere through human activities such as burning fossil fuels. In the nitrogen cycle, atmospheric nitrogen gas is converted by plants into usable forms such as ammonia and nitrates through the process of nitrogen fixation. These compounds can be used by other organisms, and nitrogen is returned to the atmosphere through denitrification and other processes. In the water cycle, the universal solvent water evaporates from land and oceans to form clouds in the atmosphere, and then precipitates back to different parts of the planet. Precipitation can seep into the ground and become part of groundwater systems used by plants and other organisms, or can runoff the surface to form lakes and rivers. Subterranean water can then seep into the ocean along with river discharges, rich with dissolved and particulate organic matter and other nutrients.

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Denitrification in the context of Woodchips

Woodchips are small- to medium-sized pieces of wood formed by cutting or chipping larger pieces of wood such as trees, branches, logging residues, stumps, roots, and wood waste.

Woodchips may be used as a biomass solid fuel and are raw material for producing wood pulp. They may also be used as an organic mulch in gardening, landscaping, and ecosystem restoration; in bioreactors for denitrification; and as a substrate for mushroom cultivation.

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Denitrification in the context of Staphylococcus aureus

Staphylococcus aureus is a Gram-positive spherically shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive for catalase and nitrate reduction and is a facultative anaerobe, meaning that it can grow without oxygen. Although S. aureus usually acts as a commensal of the human microbiota, it can also become an opportunistic pathogen, being a common cause of skin infections including abscesses, respiratory infections such as sinusitis, and food poisoning. Pathogenic strains often promote infections by producing virulence factors such as potent protein toxins, and the expression of a cell-surface protein that binds and inactivates antibodies. S. aureus is one of the leading pathogens for deaths associated with antimicrobial resistance and the emergence of antibiotic-resistant strains, such as methicillin-resistant S. aureus (MRSA). The bacterium is a worldwide problem in clinical medicine. Despite much research and development, no vaccine for S. aureus has been approved.

An estimated 21% to 30% of the human population are long-term carriers of S. aureus, which can be found as part of the normal skin microbiota, in the nostrils, and as a normal inhabitant of the lower reproductive tract of females. S. aureus can cause a range of illnesses, from minor skin infections, such as pimples, impetigo, boils, cellulitis, folliculitis, carbuncles, scalded skin syndrome, and abscesses, to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome, bacteremia, and sepsis. It is still one of the five most common causes of hospital-acquired infections and is often the cause of wound infections following surgery. Each year, around 500,000 hospital patients in the United States contract a staphylococcal infection, chiefly by S. aureus. Up to 50,000 deaths each year in the U.S. are linked to staphylococcal infection.

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Denitrification in the context of Waterlogging (agriculture)

Waterlogging water is the saturation of soil with water. Soil may be regarded as waterlogged when it is nearly saturated with water much of the time such that its air phase is restricted and anaerobic conditions prevail. In extreme cases of prolonged waterlogging, anaerobiosis occurs, the roots of mesophytes suffer, and the subsurface reducing atmosphere leads to such processes as denitrification, methanogenesis, and the reduction of iron and manganese oxides.

All plants, including crop, require air (specifically, oxygen) to respire, produce energy, and keep their cells alive. In agriculture, waterlogging typically blocks air from getting to the roots. With the exception of rice (Oryza sativa), most crops like maize and potato, are therefore highly intolerant to waterlogging. Plant cells use a variety of signals such the oxygen concentration, plant hormones like ethylene, energy and sugar status to acclimate to waterlogging-induced oxygen deprivation. Roots can survive waterlogging by forming aerenchyma, inducing anaerobic metabolism, and changing root system architecture.

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