Turfan in the context of "Dharmaguptaka"

Mahayana Buddhism entered Han China via the Silk Road, beginning in the 1st or 2nd century CE. The first documented translation efforts by Buddhist monks in China were in the 2nd century CE via the Kushan Empire into the Chinese territory bordering the Tarim Basin under Kanishka. These contacts transmitted strands of Sarvastivadan and Tamrashatiya Buddhism throughout the Eastern world.

Theravada Buddhism developed from the Pāli Canon in Sri Lanka Tamrashatiya school and spread throughout Southeast Asia. Meanwhile, Sarvastivada Buddhism was transmitted from North India through Central Asia to China. Direct contact between Central Asian and Chinese Buddhism continued throughout the 3rd to 7th centuries, much into the Tang period. From the 4th century onward, Chinese pilgrims like Faxian (395–414) and later Xuanzang (629–644) started to travel to northern India in order to get improved access to original scriptures. Between the 3rd and 7th centuries, parts of the land route connecting northern India with China was ruled by the Xiongnu, Han dynasty, Kushan Empire, the Hephthalite Empire, the Göktürks, and the Tang dynasty. The Indian form of Buddhist tantra (Vajrayana) reached China in the 7th century. Tibetan Buddhism was likewise established as a branch of Vajrayana, in the 8th century.

The Yuezhi were an ancient people first described in Chinese histories as nomadic pastoralists living in an arid grassland area in the western part of the modern Chinese province of Gansu, during the 1st millennium BC. After a major defeat at the hands of the Xiongnu in 176 BC, the Yuezhi split into two groups migrating in different directions: the Greater Yuezhi and Lesser Yuezhi. This started a complex domino effect that radiated in all directions and, in the process, set the course of history for much of Asia for centuries to come.

The Greater Yuezhi initially migrated northwest into the Ili Valley (on the modern borders of China and Kazakhstan), where they reportedly displaced elements of the Sakas. They were driven from the Ili Valley by the Wusun and migrated southward to Sogdia and later settled in Bactria. The Greater Yuezhi have consequently often been identified with peoples mentioned in classical European sources as having overrun the Greco-Bactrian Kingdom, like the Tókharoi and Asii. During the 1st century BC, one of the five major Greater Yuezhi tribes in Bactria, the Kushanas, began to subsume the other tribes and neighbouring peoples. The subsequent Kushan Empire, at its peak in the 3rd century AD, stretched from Turfan in the Tarim Basin in the north to Pataliputra on the Gangetic plain of India in the south. The Kushanas played an important role in the development of trade on the Silk Road and the introduction of Buddhism to China.

Qocho or Kara-Khoja (Chinese: 高昌回鶻; pinyin: Gāochāng Huíhú; lit. 'Gaochang Uyghurs'), also known as Idiqut ("holy wealth"; "glory"; "lord of fortune"), was a Uyghur kingdom created in 843, with strong Chinese Buddhist and Tocharian influences. It was founded by refugees fleeing the destruction of the Uyghur Khaganate after being driven out by the Yenisei Kirghiz. They made their winter capital in Qocho (also called Gaochang or Qara-Khoja, near modern Turpan) and summer capital in Beshbalik (modern Jimsar County, also known as Tingzhou). Its population is referred to as the "Xizhou Uyghurs" after the old Tang Chinese name for Gaochang, the "Qocho Uyghurs" after their capital, the "Kucha Uyghurs" after another city they controlled, or the "Arslan ("Lion") Uyghurs" after their king's title.

Buddhism in Central Asia mainly existed in Mahayana forms and was historically especially prevalent along the Silk Road. The history of Buddhism in Central Asia is closely related to the Silk Road transmission of Buddhism during the first millennium of the common era. Buddhism dominated in Pre-Islamic Central Asia. It has been argued that the spread of Indian culture and religions, especially Buddhism, as far as Sogdia, corresponded to the rule of the Kidarites over the regions from Sogdia to Gandhara.

Buddhism has now been largely replaced by Islam in modern Central Asia. Uzbekistan and Kazakhstan have the most Buddhists, largely practiced by their Koryo-saram minority, although the former has the lowest percentage of Buddhists. Due to historical Tibetan, Mongol and Manchurian influence, Kyrgyzstan has the highest percentage of Buddhists in Central Asia.

The Tocharian (sometimes Tokharian) languages (US: /toʊˈkɛəriənˌ -ˈkɑːr-/ toh-KAIR-ee-ən, -⁠KAR-; UK: /tɒˈkɑːriən/ to-KAR-ee-ən), also known as the Agni-Kuči, Agnean-Kuchean or Kuchean-Agnean languages, are an extinct branch of the Indo-European language family spoken by inhabitants of the Tarim Basin, the Tocharians. The languages are known from manuscripts dating from the 5th to the 8th century AD, which were found in oasis cities on the northern edge of the Tarim Basin (now part of Xinjiang in Northwest China) and the Lop Desert. The discovery of these languages in the early 20th century contradicted the formerly prevalent idea of an east–west division of the Indo-European language family as centum and satem languages, and prompted reinvigorated study of the Indo-European family. Scholars studying these manuscripts in the early 20th century identified their authors with the Tokharoi, a name used in ancient sources for people of Bactria (Tokharistan). Although this identification is now believed to be mistaken, "Tocharian" remains the usual term for these languages.

The discovered manuscripts record two closely related languages, called Tocharian A (also East Tocharian or Turfanian) and Tocharian B (West Tocharian or Kuchean). The subject matter of the texts suggests that Tocharian A was more archaic and used as a Buddhist liturgical language, while Tocharian B was more actively spoken in the entire area from Turfan in the east to Tumshuq in the west. A body of loanwords and names found in Prakrit documents from the Lop Nur basin have been dubbed Tocharian C (Kroränian). A claimed find of ten Tocharian C texts written in Kharosthi has been discredited.

The Subeshi culture (simplified Chinese: 苏贝希文化; traditional Chinese: 蘇貝希文化; 1100–100 BCE), also rendered as Subeishi culture or Subeixi culture, is an Iron Age culture from the area of Shanshan County, Turfan, Xinjiang, at the eastern edge of the Tarim Basin. The Subeshi culture contributes some of the later period Tarim Mummies. It might be associated with the Jushi Kingdom known from Chinese historical sources. The culture includes three closely related cemeteries:

• the Subeshi cemetery
• the Shengjindian cemetery,
• the Yanghai cemetery.

After 200 BCE, the Subeshi culture may have evolved into the later walled city-state culture of the Jushi Kingdom.