A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the instrument.
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👉 Pipette in the context of Clinical chemistry
Clinical chemistry (also known as chemical pathology, clinical biochemistry or medical biochemistry) is a division in pathology and medical laboratory sciences focusing on qualitative tests of important compounds, referred to as analytes or markers, in bodily fluids and tissues using analytical techniques and specialized instruments. This interdisciplinary field includes knowledge from medicine, biology, chemistry, biomedical engineering, informatics, and an applied form of biochemistry (not to be confused with medicinal chemistry, which involves basic research for drug development).
The discipline originated in the late 19th century with the use of simple chemical reaction tests for various components of blood and urine. Many decades later, clinical chemists use automated analyzers in many clinical laboratories. These instruments perform experimental techniques ranging from pipetting specimens and specimen labelling to advanced measurement techniques such as spectrometry, chromatography, photometry, potentiometry, etc. These instruments provide different results that help identify uncommon analytes, changes in light and electronic voltage properties of naturally occurring analytes such as enzymes, ions, electrolytes, and their concentrations, all of which are important for diagnosing diseases.
In this Dossier
Pipette in the context of Water clocks
A water clock, or clepsydra (from Ancient Greek κλεψύδρα (klepsúdra) 'pipette, water clock'; from κλέπτω (kléptō) 'to steal' and ὕδωρ (hydor) 'water'; lit. ' water thief'), is a timepiece by which time is measured by the regulated flow of liquid into (inflow type) or out from (outflow type) a vessel, and where the amount of liquid can then be measured.
Water clocks are some of the oldest time-measuring instruments. The simplest form of water clock, with a bowl-shaped outflow, existed in Babylon, Egypt, and Persia around the 16th century BC. Other regions of the world, including India and China, also provide early evidence of water clocks, but the earliest dates are less certain. Water clocks were used in ancient Greece and in ancient Rome, as described by technical writers such as Ctesibius (died 222 BC) and Vitruvius (died after 15 BC).
Pipette in the context of Eyedropper
An eye dropper, also called Pasteur pipette or simply dropper, is a device used to transfer small quantities of liquids. They are used in the laboratory and also to dispense small amounts of liquid medicines. A very common use is to dispense eye drops into the eye. The commonly recognized form is a glass tube tapered to a narrow point (a pipette) and fitted with a rubber bulb at the top, although many styles of both plastic and glass droppers exist. The combination of the pipette and rubber bulb has also been referred to as a teat pipette. The Pasteur pipette name is from the French scientist Louis Pasteur, who used a variant of them extensively during his research. In the past, there was no equipment to transfer a chemical solution without exposing it to the external environment. The hygiene and purity of chemical compounds is necessary for the expected result of each experiment. The eye dropper, both glass and plastic types, can be sterilized and plugged with a rubber bulb at the open end of the pipette preventing any contamination from the atmosphere. Generally, they are considered cheap enough to be disposable, however, so long as the glass point is not chipped, the eye dropper may be washed and reused indefinitely.