Microspectrophotometry is the measure of the spectra of microscopic samples using different wavelengths of electromagnetic radiation (e.g. ultraviolet, visible and near infrared, etc.) It is accomplished with microspectrophotometers, cytospectrophotometers, microfluorometers, Raman microspectrophotometers, etc. A microspectrophotometer can be configured to measure transmittance, absorbance, reflectance, light polarization, fluorescence (or other types of luminescence such as photoluminescence) of sample areas less than a micrometer in diameter through a modified optical microscope.
>>>PUT SHARE BUTTONS HERE<<<
👉 Micro-spectrophotometry in the context of Cytochemistry
Cytochemistry is the branch of cell biology dealing with the detection of cell constituents by means of biochemical analysis and visualization techniques. This is the study of the localization of cellular components through the use of staining methods. The term is also used to describe a process of identification of the biochemical content of cells. Cytochemistry is a science of localizing chemical components of cells and cell organelles on thin histological sections by using several techniques like enzyme localization, micro-incineration, micro-spectrophotometry, radioautography, cryo-electron microscopy, X-ray microanalysis by energy-dispersive X-ray spectroscopy, immunohistochemistry and cytochemistry, etc.