Photoluminescence in the context of "Micro-spectrophotometry"

Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance. Fluorescent materials generally cease to glow nearly immediately when the radiation source stops. This distinguishes them from the other type of light emission, phosphorescence. Phosphorescent materials continue to emit light for some time after the radiation stops.This difference in duration is a result of quantum spin effects.

Fluorescence occurs when a photon from incoming radiation is absorbed by a molecule, exciting it to a higher energy level, followed by the emission of light as the molecule returns to a lower energy state. The emitted light may have a longer wavelength and, therefore, a lower photon energy than the absorbed radiation. For example, the absorbed radiation could be in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region. This gives the fluorescent substance a distinct color, best seen when exposed to UV light, making it appear to glow in the dark. However, any light with a shorter wavelength may cause a material to fluoresce at a longer wavelength. Fluorescent materials may also be excited by certain wavelengths of visible light, which can mask the glow, yet their colors may appear bright and intensified. Other fluorescent materials emit their light in the infrared or even the ultraviolet regions of the spectrum.

Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength. Unlike fluorescence, a phosphorescent material does not immediately reemit the radiation it absorbs. Instead, a phosphorescent material absorbs some of the radiation energy and reemits it for a much longer time after the radiation source is removed.

In a general sense, there is no distinct boundary between the emission times of fluorescence and phosphorescence (i.e.: if a substance glows under a black light it is generally considered fluorescent, and if it glows in the dark it is often simply called phosphorescent). In a modern, scientific sense, the phenomena can usually be classified by the three different mechanisms that produce the light and the typical timescales at which they emit light: fluorescence, triplet phosphorescence, and persistent phosphorescence. Whereas fluorescent materials stop emitting light within nanoseconds (billionths of a second) after the excitation radiation is removed, phosphorescent materials may continue to emit an afterglow ranging from a few microseconds to many hours after the excitation is removed.

The optical properties of a material define how it interacts with light. The optical properties of matter are studied in optical physics (a subfield of optics) and applied in materials science. The optical properties of matter include:

• Refractive index
• Dispersion
• Transmittance and Transmission coefficient
• Absorption
• Scattering
• Turbidity
• Reflectance and Reflectivity (reflection coefficient)
• Albedo
• Perceived color
• Fluorescence
• Phosphorescence
• Photoluminescence
• Optical bistability
• Dichroism
• Birefringence
• Optical activity
• Photosensitivity

A basic distinction is between isotropic materials, which exhibit the same properties regardless of the direction of the light, and anisotropic ones, which exhibit different properties when light passes through them in different directions.

Quantum dots (QDs) or semiconductor nanocrystals are semiconductor particles a few nanometres in size with optical and electronic properties that differ from those of larger particles via quantum mechanical effects. They are a central topic in nanotechnology and materials science. When a quantum dot is illuminated by UV light, an electron in the quantum dot can be excited to a state of higher energy. In the case of a semiconducting quantum dot, this process corresponds to the transition of an electron from the valence band to the conduction band. The excited electron can drop back into the valence band releasing its energy as light. This light emission (photoluminescence) is illustrated in the figure on the right. The color of that light depends on the energy difference between the discrete energy levels of the quantum dot in the conduction band and the valence band.

In other words, a quantum dot can be defined as a structure on a semiconductor which is capable of confining electrons in three dimensions, enabling the ability to define discrete energy levels. The quantum dots are tiny crystals that can behave as individual atoms, and their properties can be manipulated.