Lamprey in the context of "Hagfish"

Fish began evolving about 530 million years ago during the Cambrian explosion. It was during this time that the early chordates developed the skull and the vertebral column, leading to the first craniates and vertebrates. The first fish lineages belong to the Agnatha, or jawless fish. Early examples include Haikouichthys. During the late Cambrian, eel-like jawless fish called the conodonts, and small mostly armoured fish known as ostracoderms, first appeared. Most jawless fish are now extinct; but the extant lampreys may approximate ancient pre-jawed fish. Lampreys belong to the Cyclostomata, which includes the extant hagfish, and this group may have split early on from other agnathans.

The earliest jawed vertebrates probably developed during the late Ordovician period. They are first represented in the fossil record from the Silurian by two groups of fish: the armoured fish known as placoderms, which evolved from the ostracoderms; and the Acanthodii (or spiny sharks). The jawed fish that are still extant in modern days also appeared during the late Silurian: the Chondrichthyes (or cartilaginous fish) and the Osteichthyes (or bony fish). The bony fish evolved into two separate groups: the Actinopterygii (or ray-finned fish) and Sarcopterygii (which includes the lobe-finned fish).

Agnatha (/ˈæɡnəθə, æɡˈneɪθə/; from Ancient Greek ἀ- (a-) 'without' and γνάθος (gnáthos) 'jaws') or jawless fish is a paraphyletic infraphylum of animals in the subphylum Vertebrata of the phylum Chordata, characterized by the lack of jaws. The group consists of both living (cyclostomes such as hagfishes and lampreys) and extinct clades (e.g. conodonts and cephalaspidomorphs, among others). They are sister to vertebrates with jaws known as gnathostomes, who evolved from jawless ancestors during the early Silurian by developing folding articulations in the first pairs of gill arches.

Molecular data, both from rRNA and from mtDNA as well as embryological data, strongly supports the hypothesis that both groups of living agnathans, hagfishes and lampreys, are more closely related to each other than to jawed fish, forming the superclass Cyclostomi.

Hematophagy (sometimes spelled haematophagy or hematophagia) is the practice by certain animals of feeding on blood (from the Greek words αἷμα haima "blood" and φαγεῖν phagein "to eat"). Since blood is a fluid tissue rich in nutritious proteins and lipids that can be taken without great effort, hematophagy is a preferred form of feeding for many small animals, such as worms and arthropods. Some intestinal nematodes, such as Ancylostomatids, feed on blood extracted from the capillaries of the gut, and about 75 percent of all species of leeches (e.g., Hirudo medicinalis) are hematophagous. The spider Evarcha culicivora feeds indirectly on vertebrate blood by specializing on blood-filled female mosquitoes as their preferred prey. Some fish, such as lampreys and candirus; mammals, especially vampire bats; and birds, including the vampire finch, Hood mockingbird, Tristan thrush, and oxpeckers, also practice hematophagy.

Gill slits are individual openings to gills, i.e., multiple gill arches, which lack a single outer cover. Such gills are characteristic of cartilaginous fish such as sharks and rays, as well as deep-branching vertebrates such as lampreys. In contrast, bony fishes have a single outer bony gill covering called an operculum.

Most sharks and rays have five pairs of gill slits, but a few species have 6 or 7 pairs. Shark gill slits lie in a row behind the head. The anterior edge of a gill slit is motile, moving outward to allow water to exit, but closing to prevent reverse flow. A modified slit, called a spiracle, lies just behind the eye, which assists the shark with taking in water during respiration and plays a major role in bottom–dwelling sharks. Spiracles are reduced or missing in active pelagic sharks. While the shark is moving, water passes through the mouth and over the gills in a process known as "ram ventilation". While at rest, most sharks pump water over their gills to ensure a constant supply of oxygenated water. A small number of species have lost the ability to pump water through their gills and must swim without rest. These species are obligate ram ventilators and would presumably asphyxiate if unable to move. Obligate ram ventilation is also true of some pelagic bony fish species.

Cyclostomi, often referred to as Cyclostomata (/sɪkloʊˈstɒmətə/, from Ancient Greek κύκλος (kúklos), meaning "circle", and στόμα (stóma), meaning "mouth", and thus, "circular mouth") is a superclass of vertebrates that comprises the living jawless fish classes: the lampreys and hagfishes. Both groups have jawless mouths with horny epidermal structures that function as teeth called ceratodontes, and branchial arches that are internally positioned instead of external as in the related jawed fishes.

Fish gills are organs that allow fish to breathe underwater. Most fish exchange gases like oxygen and carbon dioxide using gills on both sides of the pharynx (throat). Gills possess tissues resembling short threads, referred to as gill filaments or lamellae. Each filament contains a capillary network that provides a large surface area for exchanging oxygen and carbon dioxide. Other than respiration, these filaments have other functions including the exchange of ions, water, acids, and ammonia.

Fish respire by pulling oxygen-rich water through their mouths and pumping it over their gills. Within the gill filaments, capillary blood flows in the opposite direction to the water, causing countercurrent exchange. The gills push the oxygen-poor water out through openings in the sides of the pharynx. Some fish, like sharks and lampreys, possess multiple gill openings, but the most common group of fish alive, the bony fish, have a single gill opening on each side. This opening is hidden beneath a protective bony cover called the operculum.

The endostyle is an organ found in invertebrate chordate species of tunicates and lancelets, and in the larval stage of vertebrate lampreys. It assists in filter-feeding. It has evolved into the thyroid in vertebrate chordates.

Since the endostyle is found in all three chordate lineages, it is presumed to have arisen in the common ancestor of these taxa, along with a shift to internal feeding for extracting suspended food particles from the water. When feeding, food particles suspended in the water adhere to the mucus the endostyle produces. The filtered water is then expelled through the gill slits, while the food and mucus are swept into the esophagus by movements of the cilia that coat the endostyle.