Fluorescence microscope in the context of Optical sectioning

A microscope (from Ancient Greek μικρός (mikrós) 'small' and σκοπέω (skopéō) 'to look (at); examine, inspect') is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisible to the eye unless aided by a microscope.

There are many types of microscopes, and they may be grouped in different ways. One way is to describe the method an instrument uses to interact with a sample and produce images, either by sending a beam of light or electrons through a sample in its optical path, by detecting photon emissions from a sample, or by scanning across and a short distance from the surface of a sample using a probe. The most common microscope (and the first to be invented) is the optical microscope, which uses lenses to refract visible light that passed through a thinly sectioned sample to produce an observable image. Other major types of microscopes are the fluorescence microscope, electron microscope (both the transmission electron microscope and the scanning electron microscope) and various types of scanning probe microscopes.

An arc lamp or arc light is a lamp that produces light by an electric arc (also called a voltaic arc).

The carbon arc light, which consists of an arc between carbon electrodes in air, invented by Humphry Davy in the first decade of the 1800s, was the first practical electric light. It was widely used starting in the 1870s for street and large building lighting until it was superseded by the incandescent light in the early 20th century. It continued in use in more specialized applications where a high intensity point light source was needed, such as searchlights and movie projectors until after World War II. The carbon arc lamp is now obsolete for most of these purposes, but it is still used as a source of high intensity ultraviolet light.

Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens. The specific region an antibody recognizes on an antigen is called an epitope. Several antibodies can recognize the same epitope but differ in their binding affinity. The antibody with the higher affinity for a specific epitope will surpass antibodies with a lower affinity for the same epitope.

By conjugating the antibody to a fluorophore, the position of the target biomolecule is visualized by exciting the fluorophore and measuring the emission of light in a specific predefined wavelength using a fluorescence microscope. It is imperative that the binding of the fluorophore to the antibody itself does not interfere with the immunological specificity of the antibody or the binding capacity of its antigen.

A xenon arc lamp is a highly specialized type of gas discharge lamp, an electric light that produces light by passing electricity through ionized xenon gas at high pressure. It produces a bright white light to simulate sunlight, with applications in movie projectors in theaters, in searchlights, and for specialized uses in industry and research. For example, Xenon arc lamps and mercury lamps are the two most common lamps used in wide-field fluorescence microscopes.

The acrosome is an organelle that develops over the anterior (front) half of the head in the spermatozoa (sperm cells) of humans and many other animals. It is a cap-like structure derived from the Golgi apparatus. In placental mammals, the acrosome contains degradative enzymes (including hyaluronidase and acrosin). These enzymes break down the outer membrane of the ovum, called the zona pellucida, allowing the haploid nucleus in the sperm cell to join with the haploid nucleus in the ovum.This shedding of the acrosome, known as the acrosome reaction, can be stimulated in vitro by substances that a sperm cell may encounter naturally, such as progesterone or follicular fluid, as well as the more commonly used calcium ionophore A23187. This can be done to serve as a positive control when assessing the acrosome reaction of a sperm sample by flow cytometry or fluorescence microscopy. This is usually done after staining with a fluoresceinated lectin such as FITC-PNA, FITC-PSA, FITC-ConA, or fluoresceinated antibody such as FITC-CD46.

In the case of globozoospermia (sperm with round heads), the Golgi apparatus is not transformed into the acrosome, causing male infertility.

Prophase (from Ancient Greek προ- (pro-) 'before' and φάσις (phásis) 'appearance') is the first stage of cell division in both mitosis and meiosis. Beginning after interphase, DNA has already been replicated when the cell enters prophase. The main occurrences in prophase are the condensation of the chromatin reticulum and the disappearance of the nucleolus.