William Harvey in the context of "Franciscus Sylvius"

The history of biology traces the study of the living world from ancient to modern times. Although the concept of biology as a single coherent field arose in the 19th century, the biological sciences emerged from traditions of medicine and natural history reaching back to Ayurveda, ancient Egyptian medicine and the works of Aristotle, Theophrastus and Galen in the ancient Greco-Roman world. This ancient work was further developed in the Middle Ages by Muslim physicians and scholars such as Avicenna. During the European Renaissance and early modern period, biological thought was revolutionized in Europe by a renewed interest in empiricism and the discovery of many novel organisms. Prominent in this movement were Vesalius and Harvey, who used experimentation and careful observation in physiology, and naturalists such as Linnaeus and Buffon who began to classify the diversity of life and the fossil record, as well as the development and behavior of organisms. Antonie van Leeuwenhoek revealed by means of microscopy the previously unknown world of microorganisms, laying the groundwork for cell theory. The growing importance of natural theology, partly a response to the rise of mechanical philosophy, encouraged the growth of natural history (although it entrenched the argument from design).

Over the 18th and 19th centuries, biological sciences such as botany and zoology became increasingly professional scientific disciplines. Lavoisier and other physical scientists began to connect the animate and inanimate worlds through physics and chemistry. Explorer-naturalists such as Alexander von Humboldt investigated the interaction between organisms and their environment, and the ways this relationship depends on geography—laying the foundations for biogeography, ecology and ethology. Naturalists began to reject essentialism and consider the importance of extinction and the mutability of species. Cell theory provided a new perspective on the fundamental basis of life. These developments, as well as the results from embryology and paleontology, were synthesized in Charles Darwin's theory of evolution by natural selection. The end of the 19th century saw the fall of spontaneous generation and the rise of the germ theory of disease, though the mechanism of inheritance remained a mystery.

Marcello Malpighi (10 March 1628 – 30 November 1694) was an Italian biologist and physician, who is referred to as the "founder of microscopical anatomy, histology and father of physiology and embryology". Malpighi's name is borne by several physiological features related to the biological excretory system, such as the Malpighian corpuscles and Malpighian pyramids of the kidneys and the Malpighian tubule system of insects. The splenic lymphoid nodules are often called the "Malpighian bodies of the spleen" or Malpighian corpuscles. The botanical family Malpighiaceae is also named after him. He was the first person to see capillaries in animals, and he discovered the link between arteries and veins that had eluded William Harvey. Malpighi was one of the earliest people to observe red blood cells under a microscope, after Jan Swammerdam. His treatise De polypo cordis (1666) was important for understanding blood composition, as well as how blood clots. In it, Malpighi described how the form of a blood clot differed in the right against the left sides of the heart.

The use of the microscope enabled Malpighi to discover that insects do not use lungs to breathe, but small holes in their skin called tracheae. Malpighi also studied the anatomy of the brain and concluded this organ is a gland. In terms of modern endocrinology, this deduction is correct because the hypothalamus of the brain has long been recognized for its hormone-secreting capacity.

Jacques Dubois (Latinised as Jacobus Sylvius; 1478 – 14 January 1555) was a French anatomist. Dubois was the first to describe venous valves, although their function was later discovered by William Harvey. He was the brother of Franciscus Sylvius Ambianus (François Dubois; c. 1483 – 1536), professor of humanities at the Collège de Tournai, Paris.

ʿAlāʾ al-Dīn Abū al-Ḥasan ʿAlī ibn Abī Ḥazm al-Qarashī (Arabic: علاء الدين أبو الحسن عليّ بن أبي حزم القرشي ), known as Ibn al-Nafīs (Arabic: ابن النفيس), was an Arab polymath whose areas of work included medicine, surgery, physiology, anatomy, biology, Islamic studies, jurisprudence, and philosophy. He is known for being the first to describe the pulmonary circulation of the blood. The work of Ibn al-Nafis regarding the right sided (pulmonary) circulation pre-dates the later work (1628) of William Harvey's De motu cordis. Both theories attempt to explain circulation. The 2nd century Greek physician Galen's theory about the physiology of the circulatory system remained unchallenged until the works of Ibn al-Nafis, who has therefore been described as "the father of circulatory physiology".

As an early anatomist, Ibn al-Nafis also performed several human dissections during the course of his work, making several important discoveries in the fields of physiology and anatomy. Besides his famous discovery of the pulmonary circulation, he also gave an early insight of the coronary and capillary circulations. He was also appointed as the chief physician at al-Naseri Hospital founded by Sultan Saladin. Due to his discoveries, he has been described by some as “the second Avicenna”.