Darwin Harbour is a body of water close to the city of Darwin in the Northern Territory of Australia. It is the traditional waterways of the Larrakia people.
It opens to the north at a line from Charles Point in the west to Lee Point in the east into the Beagle Gulf and connects via the Clarence Strait with the Van Diemen Gulf. It contains Port Darwin, which is flanked by Frances Bay to the east and Cullen Bay to the west.