Pap test in the context of Anna Marion Hilliard

Anatomical pathology (Commonwealth) or anatomic pathology (U.S.) is a medical specialty that is concerned with the diagnosis of disease based on the macroscopic, microscopic, biochemical, immunologic and molecular examination of organs and tissues. Over the 20th century, surgical pathology has evolved tremendously: from historical examination of whole bodies (autopsy) to a more modernized practice, centered on the diagnosis and prognosis of cancer to guide treatment decision-making in oncology. Its modern founder was the Italian scientist Giovanni Battista Morgagni from Forlì.

Anatomical pathology is one of two branches of pathology, the other being clinical pathology, the diagnosis of disease through the laboratory analysis of bodily fluids or tissues. Often, pathologists practice both anatomical and clinical pathology, a combination known as general pathology. Similar specialties exist in veterinary pathology.

Cervical cancer screening is a medical screening test designed to identify risk of cervical cancer. Cervical screening may involve looking for viral DNA, and/or identifying abnormal, potentially precancerous cells within the cervix as well as cells that have progressed to early stages of cervical cancer. One goal of cervical screening is to allow for intervention and treatment so abnormal lesions can be removed before progression to cancer. An additional goal is to decrease mortality from cervical cancer by identifying cancerous lesions in their early stages and providing treatment before progression to more invasive disease.

Currently available screening tests fall into three categories: molecular, cytologic, and visual inspection. Molecular screening tests include nucleic acid amplification tests (NAAT), which identify high-risk human papillomavirus (HPV) strains. Cytologic tests include conventional Pap smear and liquid-based cytology. Visual Inspection tests involve application of a solution to enhance identification of abnormal areas and can utilize the naked eye or a colposcope/magnifying camera.

Georgios Nikolaou Papanikolaou (or George Papanicolaou /ˌpæpəˈnɪkəlaʊ/; Greek: Γεώργιος Ν. Παπανικολάου [papanikoˈlau]; 13 May 1883 – 19 February 1962) was a Greek physician, zoologist and microscopist who was a pioneer in cytopathology and early cancer detection, and inventor of the pap smear for detection of cervical cancer.

After studying medicine in Greece and Germany, in 1913 he emigrated to the United States and was on the faculty at Cornell Medical College. He first reported that uterine cancer cells could be detected in vaginal smears in 1928, but his work was not widely recognized until the 1940s. An extensive trial of his techniques was carried out in the early 1950s. In 1961 he was invited to the University of Miami to lead and develop there the Papanicolaou Cancer Research Institute.

Papanicolaou stain (also Papanicolaou's stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis. Although most notable for its use in the detection of cervical cancer in the Pap test or Pap smear, it is also used to stain non-gynecological specimen preparations from a variety of bodily secretions and from small needle biopsies of organs and tissues. Papanicolaou published three formulations of this stain in 1942, 1954, and 1960.