Orthochromatic in the context of "Staining"

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⭐ Core Definition: Orthochromatic

In chemistry, orthochromasia is the property of a dye or stain to not change color on binding to a target, as opposed to metachromatic stains, which do change color. The word is derived from the Greek orthos (correct, upright), and chromatic (color). Toluidine blue is an example of a partially orthochromatic dye, as it stains nucleic acids by its orthochromatic color (blue), but stains mast cell granules in its metachromatic color (red).

In spectral terms, orthochromasia refers to maintaining the position of spectral peaks, while metachromasia refers to a shift in wavelength, becoming either shorter or longer.

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Orthochromatic in the context of Photographic film

Photographic film is a strip or sheet of transparent film base coated on one side with a gelatin emulsion containing microscopically small light-sensitive silver halide crystals. The sizes and other characteristics of the crystals determine the sensitivity, contrast, and resolution of the film. Film is typically segmented in frames, that give rise to separate photographs.

The emulsion will gradually darken if left exposed to light, but the process is too slow and incomplete to be of any practical use. Instead, a very short exposure to the image formed by a camera lens is used to produce only a very slight chemical change, proportional to the amount of light absorbed by each crystal. This creates an invisible latent image in the emulsion, which can be chemically developed into a visible photograph. In addition to visible light, most films are sensitive to ultraviolet light, X-rays, gamma rays, and high-energy particles. Unmodified silver halide crystals are sensitive only to the blue part of the visible spectrum, producing unnatural-looking renditions of some colored subjects. This problem was resolved with the discovery that certain dyes, called sensitizing dyes, when adsorbed onto the silver halide crystals made them respond to other colors as well. First orthochromatic (sensitive to blue and green) and finally panchromatic (sensitive to all visible colors) films were developed. Panchromatic film renders all colors in shades of gray approximately matching their subjective brightness. By similar techniques, special-purpose films can be made sensitive to the infrared (IR) region of the spectrum.

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