Middlesboro (locally /ˈmɪdəlzbʌrə/) is a home rule-class city in Bell County, Kentucky, United States. The population was 10,334 at the 2010 U.S. census, while its micropolitan area had a population of 69,060.
It is located 1 mile (1.6 km) west of the Cumberland Gap and is the largest city in southeastern Kentucky. It is located entirely between Pine Mountain and the Cumberland Mountains in the Middlesboro Basin, an enormous meteorite crater (one of three known astroblemes in the state).
U.S. Route 25E (US 25E) is the eastern branch of US 25 from Newport, Tennessee, where US 25 splits into US 25E and US 25W, to North Corbin, Kentucky, where the two highways rejoin. The highway, however, continues as US 25E for roughly two miles (3.2 km) until it joins Interstate 75 (I-75) in the Laurel County community of North Corbin at exit 29. The highway serves the Appalachia regions of Kentucky's Cumberland Plateau and the Ridge-and-Valley section of East Tennessee, including the urbanized areas of Corbin and Middlesboro in Kentucky and Morristown in Tennessee.
US 25E follows the original pathway of early U.S. pioneer Daniel Boone's Wilderness Road, which contributed to the increased settlement of Appalachia given its access through the rugged Cumberland Gap. By 1815, the route became the first state-funded road in Tennessee and, in 1923, unsigned State Route 32 (SR 32) in the Tennessee State Route System in its statewide entirety. It would be recognized as part of the Dixie Highway, one of the earliest federal auto trails, in 1915. The route was officially established as US 25E with the creation of the U.S. Numbered Highway System in 1926.