Microbialite is a benthic sedimentary deposit made of carbonate mud (particle diameter less than 5 μm) that is formed with the mediation of microbes. The constituent carbonate mud is a type of automicrite (or authigenic carbonate mud); therefore, it precipitates in situ instead of being transported and deposited. Being formed in situ, a microbialite can be seen as a type of boundstone where reef builders are microbes, and precipitation of carbonate is biotically induced instead of forming tests, shells or skeletons.
Microbialites can also be defined as microbial mats with lithification capacity. Bacteria can precipitate carbonate both in shallow and in deep water (except for Cyanobacteria) and so microbialites can form regardless of the sunlight.