Luigi Rolando (16 June 1773, Turin – 20 April 1831, Turin) was an Italian anatomist known for his pioneering research in brain localization of function.
He studied medicine in Turin, later continuing his education in Florence, where he studied engraving, drawing, anatomical dissection, and conducted microscopic investigations of nerve tissue. From 1804 he was a professor at the University of Sassari, and in 1814 was appointed professor of anatomy at the University of Turin.