Gladius (cephalopod) in the context of "Myopsida"

A squid (pl. squid) is a mollusc with an elongated soft body, large eyes, eight arms, and two tentacles in the orders Myopsida, Oegopsida, and Bathyteuthida (though many other molluscs within the broader Neocoleoidea are also called squid despite not strictly fitting these criteria). Like all other cephalopods, squid have a distinct head, bilateral symmetry, and a mantle. They are mainly soft-bodied, like octopuses, but have a small internal skeleton in the form of a rod-like gladius or pen, made of chitin.

Squid diverged from other cephalopods during the Jurassic and radiated at the beginning of the Late Cretaceous, and occupy a similar role to teleost fish as open-water predators of similar size and behaviour. They play an important role in the open-water food web. The two long tentacles are used to grab prey and the eight arms to hold and control it. The beak then cuts the food into suitable size chunks for swallowing. Squid are rapid swimmers, moving by jet propulsion, and largely locate their prey by sight. They are among the most intelligent of invertebrates, with groups of Humboldt squid having been observed hunting cooperatively. They are preyed on by sharks, other fish, sea birds, seals and cetaceans, particularly sperm whales.

Chitin (C₈H₁₃O₅N)_n (/ˈkaɪtɪn/ KY-tin) is a long-chain polymer of N-acetylglucosamine, an amide derivative of glucose. Chitin is the second most abundant polysaccharide in nature (behind only cellulose); an estimated 1 billion tons of chitin are produced each year in the biosphere. It is a primary component of cell walls in fungi (especially filamentous and mushroom-forming fungi), the exoskeletons of arthropods such as crustaceans and insects, the radulae, cephalopod beaks and gladii of molluscs and in some nematodes and diatoms.It is also synthesised by at least some fish and lissamphibians. Commercially, chitin is extracted from the shells of crabs, shrimps, shellfish and lobsters, which are major by-products of the seafood industry. The structure of chitin is comparable to cellulose, forming crystalline nanofibrils or whiskers. It is functionally comparable to the protein keratin. Chitin has proved useful for several medicinal, industrial and biotechnological purposes.

An endoskeleton (from Ancient Greek ἔνδον (éndon), meaning "inside", and σκελετός (skeletós), meaning "skeleton") is a structural frame (skeleton) — usually composed of mineralized tissue — on the inside of an animal, overlaid by soft tissues. Endoskeletons serve as structural support against gravity and mechanical loads, and provide anchoring attachment sites for skeletal muscles to transmit force and allow movements and locomotion.

Vertebrates and the closely related cephalochordates are the predominant animal clade with endoskeletons (made of mostly bone and sometimes cartilage, as well as notochordal glycoprotein and collagen fibers), although invertebrates such as sponges also have evolved a form of "rebar" endoskeletons made of diffuse meshworks of calcite/silica structural elements called spicules, and echinoderms have a dermal calcite endoskeleton known as ossicles. Some coleoid cephalopods (squids and cuttlefish) have an internalized vestigial aragonite/calcite-chitin shell known as gladius or cuttlebone, which can serve as muscle attachments but the main function is often to maintain buoyancy rather than to give structural support, and their body shape is largely maintained by hydroskeleton.

Coleoidea or Dibranchiata is one of the two subclasses of cephalopod molluscs containing all the various taxa popularly thought of as "soft-bodied" or "shell-less" (i.e. octopus, squid and cuttlefish). Unlike its sister groups, the shelled †Ammonoidea and Nautiloidea, the coleoids have at most an internal shell called cuttlebone or gladius that is used for buoyancy or as muscle attachment. Some species, notably the incirrate octopuses (including commonly known varieties living in the shallows), have lost their internal shell altogether, while in some it has been replaced by a chitinous support structure.

Cuttlebone, also known as cuttlefish bone, is a hard, brittle internal structure (an internal shell) found in all members of the family Sepiidae, commonly known as cuttlefish, within the cephalopods. In other cephalopod families it is called a gladius.

Cuttlebone is composed primarily of aragonite. It is a chambered structure that the animal can fill with gas or liquid for buoyancy control. On the ventral (bottom) side of the cuttlebone is the highly modified siphuncle; this is the organ with which the cuttlebone is filled with gas or liquid. The microscopic structure of cuttlebone consists of narrow layers connected by numerous upright pillars.