Interstate 64 (I-64) in the US state of Kentucky travels for 191 miles (307 km), passing by the major towns and cities of Louisville, Frankfort, Lexington, and Ashland. It has several major junctions with other Interstates, including I-65, I-71, I-264, and I-265 in Louisville and I-75 in Lexington.
The portion of I-64 in Kentucky is host to two "exceptionally significant" structures indicated by the Federal Highway Administration (FHWA). One is the Cochran Hill Tunnel, a twin tube at Cherokee Park in Louisville built in 1974, and the other is a 1960s-era modern-styled rest area near Winchester.