Annelie Lotriet (born 8 June 1960) is a South African politician who is currently serving as the Deputy Speaker of the National Assembly of South Africa since June 2024. She has served in the Official Opposition Shadow Cabinet since she joined the National Assembly in 2009. She is also a Deputy Chairperson of the Democratic Alliance (DA) Federal Council.
Born in Johannesburg, Lotriet spent the first two decades of her career as an academic in the Free State, teaching at Vista University and then at the University of the Free State. After one term as a local councillor in Mangaung, she entered politics full-time at the 2009 general election. She went on to serve as Shadow Minister of Arts and Culture from 2009 to 2012, Shadow Minister of Higher Education and Training from 2012 to 2014, Shadow Minister of Science and Technology from 2014 to 2019, and DA Caucus Chairperson from 2019 to 2022. Most recently, she was Opposition Deputy Chief Whip from August 2022 to June 2024.