Genetically modified organism in the context of "Genetic engineering"

Organic farming, also known as organic agriculture or ecological farming or biological farming, is an agricultural system that emphasizes the use of naturally occurring, non-synthetic inputs, such as compost manure, green manure, and bone meal and places emphasis on techniques such as crop rotation, companion planting, and mixed cropping. Biological pest control methods such as the fostering of insect predators are also encouraged. Organic agriculture can be defined as "an integrated farming system that strives for sustainability, the enhancement of soil fertility and biological diversity while, with rare exceptions, prohibiting synthetic pesticides, antibiotics, synthetic fertilizers, genetically modified organisms, and growth hormones". It originated early in the 20th century in reaction to rapidly changing farming practices. Certified organic agriculture accounted for 70 million hectares (170 million acres) globally in 2019, with over half of that total in Australia.

Organic standards are designed to allow the use of naturally occurring substances while prohibiting or severely limiting synthetic substances. For instance, naturally occurring pesticides, such as garlic extract, bicarbonate of soda, or pyrethrin (which is found naturally in the Chrysanthemum flower), are permitted, while synthetic fertilizers and pesticides, such as glyphosate, are prohibited. Synthetic substances that are allowed only in exceptional circumstances may include copper sulfate, elemental sulfur, and veterinary drugs. Genetically modified organisms, nanomaterials, human sewage sludge, plant growth regulators, hormones, and antibiotic use in livestock husbandry are prohibited. Broadly, organic agriculture is based on the principles of health, care for all living beings and the environment, ecology, and fairness. Organic methods champion sustainability, self-sufficiency, autonomy and independence, health, animal welfare, food security, and food safety. It is often seen as part of the solution to the impacts of climate change.

Genetic engineering techniques allow the modification of animal and plant genomes. Techniques have been devised to insert, delete, and modify DNA at multiple levels, ranging from a specific base pair in a specific gene to entire genes. There are a number of steps that are followed before a genetically modified organism (GMO) is created. Genetic engineers must first choose what gene they wish to insert, modify, or delete. The gene must then be isolated and incorporated, along with other genetic elements, into a suitable vector. This vector is then used to insert the gene into the host genome, creating a transgenic or edited organism.

The ability to genetically engineer organisms is built on years of research and discovery on gene function and manipulation. Important advances included the discovery of restriction enzymes, DNA ligases, and the development of polymerase chain reaction and sequencing.

Serbian cuisine (Serbian: српска кухиња, romanized: srpska kuhinja) is a Balkan cuisine that consists of the culinary methods and traditions of Serbia. Its roots lie in Serbian history, including centuries of cultural contact and influence with the Greeks and the Byzantine Empire, the Ottoman Empire, and Serbia's Balkan neighbours, especially during the existence of Yugoslavia. Historically, Serbian food develops from pastoral customs that involved the keeping of sheep in mountain highlands, in a climate and regional context that favoured animal husbandry over vegetable farming; Serbian food is therefore traditionally richer in animal products and basic grains—corn, wheat and oats—than fresh vegetable dishes. Following the abandonment of widely practiced pastoral lifestyles, Serbian food emerged through the Middle Ages heavily dependent not on lamb or mutton, but on the keeping of pigs for the annual cull and the production of various cured meats, such as sausages, bacon and ham products.

The Serbian government has passed laws banning the production and import of genetically modified foods, a legislative decision which has been applauded by environmentalists but caused a long-running dispute with the World Trade Organization, preventing Serbia from being able to join the WTO.

Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.

In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMOs). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large number of bacteria, each of which contains copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as "clones". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them. The idea arose that different DNA sequences could be inserted into a plasmid and that these foreign sequences would be carried into bacteria and replicated as part of the plasmid. That is, these plasmids could serve as cloning vectors to carry genes.

Cisgenesis is a product designation for a category of genetically engineered plants. A variety of classification schemes have been proposed that order genetically modified organisms based on the nature of introduced genotypical changes, rather than the process of genetic engineering.

Cisgenesis (etymology: cis = same side; and genesis = origin) is one term for organisms that have been engineered using a process in which genes are artificially transferred between organisms that could otherwise be conventionally bred. Genes are only transferred between closely related organisms. Nucleic acid sequences must be isolated and introduced using the same technologies that are used to produce transgenic organisms, making cisgenesis similar in nature to transgenesis. The term was first introduced in 2000 by Henk J. Schouten and Henk Jochemsen, and in 2004 a PhD thesis by Jan Schaart of Wageningen University in 2004, discussing making strawberries less susceptible to Botrytis cinerea.

Genetically modified fish (GM fish) are organisms from the taxonomic clade which includes the classes Agnatha (jawless fish), Chondrichthyes (cartilaginous fish) and Osteichthyes (bony fish) whose genetic material (DNA) has been altered using genetic engineering techniques. In most cases, the aim is to introduce a new trait to the fish which does not occur naturally in the species, i.e. transgenesis.

GM fish are used in scientific research and kept as pets. They are being developed as environmental pollutant sentinels and for use in aquaculture food production. In 2015, the AquAdvantage salmon was approved by the US Food and Drug Administration (FDA) for commercial production, sale and consumption, making it the first genetically modified animal to be approved for human consumption. Some GM fish that have been created have promoters driving an over-production of "all fish" growth hormone. This results in dramatic growth enhancement in several species, including salmonids, carps and tilapias.

The zebrafish (Danio rerio) is a species of freshwater ray-finned fish belonging to the family Danionidae of the order Cypriniformes. Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a "tropical fish" although it is both tropical and subtropical).

The zebrafish is an important and widely used vertebrate model organism in scientific research, particularly developmental biology, but also gene function, oncology, teratology, and drug development, in particular pre-clinical development, due to its scalability from high numbers of offspring and ease of drug delivery through water into the gills. It is also notable for its regenerative abilities, and has been modified by researchers to produce many transgenic strains.