Sheldon Moldoff in the context of "Ace the Bat-Hound"

Batgirl is the name of several superheroines appearing in American comic books published by DC Comics, depicted as female counterparts and allies to the superhero Batman. The character Betty Kane was introduced into publication in 1961 by Bill Finger and Sheldon Moldoff as Bat-Girl, and was replaced in 1967 by Barbara Gordon, who became the most iconic Batgirl. The character debuted in Detective Comics #359 (January 1967) by writer Gardner Fox and artist Carmine Infantino, introduced as the niece/adoptive daughter of police commissioner James Gordon.

Batgirl operates in Gotham City, allying herself with Batman and the original Robin, Dick Grayson, along with other masked vigilantes. The character appeared regularly in Detective Comics, Batman Family, and several other books produced by DC until 1988. That year, Barbara Gordon appeared in Barbara Kesel's Batgirl Special #1, in which she retires from crime-fighting. She subsequently appeared in Alan Moore's graphic novel Batman: The Killing Joke where, in her civilian identity, she is shot by the Joker and left paraplegic. Although she is reimagined as the computer expert and information broker Oracle by editor Kim Yale and writer John Ostrander the following year, her paralysis sparked debate about the portrayal of women in comics, particularly violence depicted toward female characters.

The Scarecrow is a supervillain appearing in American comic books published by DC Comics. Created by writer Bill Finger and artist Bob Kane, the character first appeared in World's Finest Comics #3 (1941) as an adversary of the superhero Batman. Although the Scarecrow only made two appearances in the 1940s, he was revived by writer Gardner Fox and artist Sheldon Moldoff in Batman #189 (1967). The character has since become one of Batman's most enduring enemies, belonging to the collective of adversaries that make up his rogues gallery.

In the DC Universe, the Scarecrow is the alias of Jonathan Crane, a professor of psychology turned criminal mastermind. Abused and bullied in his youth, he becomes obsessed with fear and develops a hallucinogenic drug—dubbed "fear toxin"—to terrorize Gotham City and exploit the phobias of its protector, Batman. As the self-proclaimed "Master of Fear", the Scarecrow's crimes do not stem from a common desire for wealth or power, but from a sadistic pleasure in subjecting others to his experiments on the manipulation of fear. An outfit symbolic of his namesake with a stitched burlap mask serves as the Scarecrow's visual motif.

Poison Ivy is a character appearing in American comic books published by DC Comics. Created by writer Robert Kanigher, Sheldon Moldoff and artist Carmine Infantino, she debuted in Batman #181 (June 1966) and has become one of the superhero Batman's most enduring enemies belonging to the collective of adversaries that make up his rogues gallery.

In her comic book appearances, Poison Ivy is depicted as a doctor of botany-turned-misanthropic ecoterrorist in Gotham City named Pamela Lillian Isley, PhD (/ˈaɪzli/ EYEZ-lee) with the ability to control all plant life. Empowered by an elemental force known as the "Green", Ivy attempts to protect the sanctity and supremacy of nature at all costs by lashing out against humanity, which brings her into conflict with Batman. While usually portrayed as a supervillain, Ivy has also been an antiheroine at times as well as the primary love interest of Harley Quinn as of The New 52 and DC Rebirth relaunches. A one-piece costume adorned with leaves and vines serves as Poison Ivy's visual motif.