Santafé de Bogotá in the context of Real Audiencia

Gonzalo Jiménez de Quesada y Rivera, also spelled as Ximénez and De Quezada, (Spanish: [gonˈθalo xiˈmeneθ ðe keˈsaða]; 1509 – 16 February 1579) was a Spanish explorer and conquistador in northern South America, territories currently known as Colombia. He explored the territory named by him, New Kingdom of Granada, and founded its capital, Santafé de Bogotá. As a well-educated lawyer he was one of the intellectuals of the Spanish conquest. He was an effective organizer and leader, designed the first legislation for the government of the area, and was its historian. He was governor of Cartagena between 1556 and 1557, and after 1569 he undertook explorations toward the east, searching for the elusive El Dorado. The campaign didn't succeed and Jiménez then returned to New Granada in 1573. He has been suggested as a possible model for Cervantes' Don Quixote.

Hernán Pérez de Quesada, sometimes spelled as Quezada, (c. 1515 – 1544) was a Spanish conquistador. Second in command of the army of his elder brother, Gonzalo Jiménez de Quesada, Hernán was part of the first European expedition towards the inner highlands of the Colombian Andes. The harsh journey, taking almost a year and many deaths, led through the modern departments Magdalena, Cesar, Santander, Boyacá, Cundinamarca and Huila of present-day Colombia between 1536 and 1539 and, without him, Meta, Caquetá and Putumayo of Colombia and northern Peru and Ecuador between 1540 and 1542.

Hernán founded Sutatausa, Cundinamarca, and aided in the conquest of various indigenous groups, such as the Chimila, Muisca, Panche, Lache, U'wa, Sutagao and others. Under the command of Hernán Pérez de Quesada the last independent Muisca ruler; hoa Quiminza was publicly decapitated. As second in command under his brother, in the previous years psihipquias Tisquesusa and Sagipa and Tundama of Duitama had suffered a similar fate. After returning from his expeditions to the south reaching Quito, where he reunited with his younger brother Francisco, both De Quesadas went back to Santafé de Bogotá. Hernán was tried and imprisoned there for the murders of the Muisca rulers by the governor of the capital of the New Kingdom of Granada. In 1544, en route to Cartagena with his brother Francisco, their ship was hit by lightning off the coast of Cabo de la Vela in the Caribbean Sea killing Hernán and Francisco and wounding several other conquistadors.

Tunja (Spanish pronunciation: [ˈtuŋxa]) is a municipality and city on the Eastern Ranges of the Colombian Andes, in the region known as the Altiplano Cundiboyacense, 130 km northeast of Bogotá. In 2018 the municipality had a population of 172,548. It is the capital of Boyacá department and the Central Boyacá Province. Tunja is an important educational centre of well-known universities. In the time before the Spanish conquest of the Muisca, there was an indigenous settlement, called Hunza, seat of the hoa Eucaneme, conquered by the Spanish conquistadors on August 20, 1537. The Spanish city was founded by captain Gonzalo Suárez Rendón on August 6, 1539, exactly one year after the capital Santafé de Bogotá. The city hosts the most remaining Muisca architecture: Hunzahúa Well, Goranchacha Temple and Cojines del Zaque.

Tunja is a tourist destination, especially for religious colonial architecture, with the Casa Fundador Gonzalo Suárez Rendón recognized as the oldest remnant. In addition to its religious and historical sites it is host to several internationally known festivals and is a jumping-off point for regional tourist destinations such as Villa de Leyva, Paipa, and Sierra Nevada del Cocuy. It is a stop on the Pan American Highway which connects Tunja to Bogotá and Santa Marta and eventually to the northern and southernmost parts of South America.