Oxygen-18 in the context of Marine isotope stage

Marine isotope stages (MIS), marine oxygen-isotope stages, or oxygen isotope stages (OIS), are alternating warm and cool periods in the Earth's paleoclimate, deduced from oxygen isotope data derived from deep sea core samples. Working backwards from the present, which is MIS 1 in the scale, stages with even numbers have high levels of oxygen-18 and represent cold glacial periods, while the odd-numbered stages are lows in the oxygen-18 figures, representing warm interglacial intervals. The data are derived from pollen and foraminifera (plankton) remains in drilled marine sediment cores, sapropels, and other data that reflect historic climate; these are called proxies.

The MIS timescale was developed from the pioneering work of Cesare Emiliani in the 1950s, and is now widely used in archaeology and other fields to express dating in the Quaternary period (the last 2.6 million years), as well as providing the fullest and best data for that period for paleoclimatology or the study of the early climate of the Earth, representing "the standard to which we correlate other Quaternary climate records". Emiliani's work in turn depended on Harold Urey's prediction in a paper of 1947 that the ratio between oxygen-18 and oxygen-16 isotopes in calcite, the main chemical component of the shells and other hard parts of a wide range of marine organisms, should vary depending on the prevailing water temperature in which the calcite was formed.

A dark nebula or absorption nebula is a type of interstellar cloud, particularly molecular clouds, that is so dense that it obscures the visible wavelengths of light from objects behind it, such as background stars and emission or reflection nebulae. The extinction of the light is caused by interstellar dust grains in the coldest, densest parts of molecular clouds. Clusters and large complexes of dark nebulae are associated with Giant Molecular Clouds. Isolated small dark nebulae are called Bok globules. Like other interstellar dust or material, the things it obscures are visible only using radio waves in radio astronomy or infrared in infrared astronomy.

Dark clouds appear so because of sub-micrometre-sized dust particles, coated with frozen carbon monoxide and nitrogen, which effectively block the passage of light at visible wavelengths. Also present are molecular hydrogen, atomic helium, CO (CO with oxygen as the O isotope), CS, NH₃ (ammonia), H₂CO (formaldehyde), c-C₃H₂ (cyclopropenylidene) and a molecular ion N₂H (diazenylium), all of which are relatively transparent.