Optical microscopy in the context of "Optical resolution"

Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born British biophysicist and Nobel laureate whose research spanned multiple areas of physics and biophysics, contributing to the scientific understanding of phosphorescence, isotope separation, optical microscopy, and X-ray diffraction. He is most noted for initiating and leading early X-ray diffraction studies on DNA at King's College London, and for his pivotal role in enabling the discovery of the double helix structure of DNA.

Wilkins began investigating nucleic acids in 1948. By 1950, he and his team had produced some of the first high-quality X-ray diffraction images of DNA fibers. He presented this work in 1951 at a conference in Naples, where it significantly influenced James Watson, prompting Watson to pursue DNA structure research with Francis Crick.

Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polariser oriented at 90 degrees to the illumination. More complex microscopy techniques which take advantage of polarized light include differential interference contrast microscopy and interference reflection microscopy. Scientists will often use a device called a polarizing plate to convert natural light into polarized light.

These illumination techniques are most commonly used on birefringent samples where the polarized light interacts strongly with the sample and so generating contrast with the background. Polarized light microscopy is used extensively in optical mineralogy.