In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light. By incorporating index of refraction in its definition, NA has the property that it is constant for a beam as it goes from one material to another, provided there is no refractive power at the interface (e.g., a flat interface). The exact definition of the term varies slightly between different areas of optics. Numerical aperture is commonly used in microscopy to describe the acceptance cone of an objective (and hence its light-gathering ability and resolution), and in fiber optics, in which it describes the range of angles within which light that is incident on the fiber will be transmitted along it.
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👉 Numerical aperture in the context of Diffraction-limited system
In optics, any optical instrument or system – a microscope, telescope, or camera – has a principal limit to its resolution due to the physics of diffraction. An optical instrument is said to be diffraction-limited if it has reached this limit of resolution performance. Other factors may affect an optical system's performance, such as lens imperfections or aberrations, but these are caused by errors in the manufacture or calculation of a lens, whereas the diffraction limit is the maximum resolution possible for a theoretically perfect, or ideal, optical system.
The diffraction-limited angular resolution, in radians, of an instrument is proportional to the wavelength of the light being observed, and inversely proportional to the diameter of its objective's entrance aperture. For telescopes with circular apertures, the size of the smallest feature in an image that is diffraction limited is the size of the Airy disk. As one decreases the size of the aperture of a telescopic lens, diffraction proportionately increases. At small apertures, such as f/22, most modern lenses are limited only by diffraction and not by aberrations or other imperfections in the construction.
Numerical aperture in the context of F-number
An f-number is a measure of the light-gathering ability of an optical system such as a camera lens. It is defined as the ratio of the system's focal length to the diameter of the entrance pupil ("clear aperture"). The f-number is also known as the focal ratio, f-ratio, or f-stop, and it is key in determining the depth of field, diffraction, and exposure of a photograph. The f-number is dimensionless and is usually expressed using a lower-case hooked f with the format f/N, where N is the f-number.
The f-number is also known as the inverse relative aperture, because it is the inverse of the relative aperture, defined as the aperture diameter divided by the focal length. A lower f-number means a larger relative aperture and more light entering the system, while a higher f-number means a smaller relative aperture and less light entering the system. The f-number is related to the numerical aperture (NA) of the system, which measures the range of angles over which light can enter or exit the system. The numerical aperture takes into account the refractive index of the medium in which the system is working, while the f-number does not.
Numerical aperture in the context of Dark field microscopy
Dark-field microscopy, also called dark-ground microscopy, describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. Consequently, the field around the specimen (i.e., where there is no specimen to scatter the beam) is generally dark.
In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but only if they have an adjustable diaphragm, which reduces the NA. Often these objective lenses have a NA that is variable from 0.7 to 1.25.
Numerical aperture in the context of Oil immersion lens
In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image. Air has a very different index of refraction from glass, making for a larger bend compared to oil, which has an index more similar to glass. Specially manufactured oil can have nearly exactly the same refractive index as glass, making an oil-immersed lens nearly as effective as having glass entirely around the sample (which would be impractical).