Night-blooming cereus in the context of Hylocereus undatus

Pollination is the transfer of pollen from an anther of a plant to the stigma of a plant, later enabling fertilisation and the production of seeds. Pollinating agents can be animals such as insects, for example bees, beetles or butterflies; birds, and bats; water; wind; and even plants themselves. Pollinating animals travel from plant to plant carrying pollen on their bodies in a vital interaction that allows the transfer of genetic material critical to the reproductive system of most flowering plants. Self-pollination occurs within a closed flower. Pollination often occurs within a species. When pollination occurs between species, it can produce hybrid offspring in nature and in plant breeding work.

In angiosperms, after the pollen grain (gametophyte) has landed on the stigma, it germinates and develops a pollen tube which grows down the style until it reaches an ovary. Its two gametes travel down the tube to where the gametophyte(s) containing the female gametes are held within the carpel. After entering an ovule through the micropyle, one male nucleus fuses with the polar bodies to produce the endosperm tissues, while the other fuses with the egg cell to produce the embryo. Hence the term: "double fertilisation". This process would result in the production of a seed, made of both nutritious tissues and embryo.

Peniocereus is a genus of vining cacti, comprising about 18 species, found from the southwestern United States and Mexico. They have a large underground tuber, thin and inconspicuous stems.Its name comes from the prefix penio- (from the Latin penis, meaning ‘tail’) and Cereus, the large genus from which it was split.

Known as the desert night-blooming cereus, it also shares its common names of "night-blooming cereus" and "queen of the night" with many other similar cacti.

Selenicereus, from Ancient Greek σελήνη (selḗnē), meaning "moon", and Latin cēreus, meaning "candle", sometimes known as moonlight cactus, is a genus of epiphytic, lithophytic, and terrestrial cacti, found in Mexico, Central America, the Caribbean and northern South America. The term night-blooming cereus is also sometimes used, but this is also used for many night-blooming cacti, including Epiphyllum and Peniocereus. In 2017, the genus Hylocereus was brought into synonymy with Selenicereus. A number of species of Selenicereus produce fruit that is eaten. The fruit, known as pitaya or pitahaya in Spanish or as dragon fruit, may be collected from the wild or the plants may be cultivated.

Selenicereus grandiflorus is a cactus species originating from the Antilles, Mexico and Central America. The species is commonly referred to as queen of the night, night-blooming cereus (though these two terms are also used for other species), large-flowered cactus, sweet-scented cactus or vanilla cactus. The true species is extremely rare in cultivation. Most of the plants under this name belong to other species or hybrids. It is often confused with the genus Epiphyllum.