Mole salamander in the context of List of U.S. state amphibians

Several groups of tetrapods have undergone secondary aquatic adaptation, an evolutionary transition from being purely terrestrial to living at least partly aquatic. These animals are called "secondarily aquatic" because although all tetrapods descended from freshwater lobe finned fish (see evolution of tetrapods), their more recent ancestors are terrestrial vertebrates that evolved on land for hundreds of millions of years, and their clades only re-adapted to aquatic environment much later.

Unlike primarily aquatic vertebrates (i.e. fish), secondarily aquatic tetrapods (especially aquatic amniotes), while having appendages such as flippers, dorsal fin and tail fins (flukes) that resemble fish fins due to convergent evolution, still have physiology based on their terrestrial ancestry, most notably their air-breathing respiration via lungs (instead of aquatic respiration via gills) and excretion of nitrogenous waste as urea or uric acid (instead of ammonia like most fish). Nearly all extant aquatic tetrapods are secondarily aquatic, with only larval amphibians (tadpoles) being primarily aquatic with gills, and only some species of paedomorphic mole salamanders (most notably the fully aquatic axolotl) retain the gill-based physiology into adulthood.

A fossorial animal (from Latin fossor 'digger') is one that is adapted to digging and which lives primarily (but not solely) underground. Examples of fossorial vertebrates are moles, badgers, naked mole-rats, meerkats, armadillos, wombats, and mole salamanders. Among invertebrates, many molluscs (e.g., clams), insects (e.g., beetles, wasps, bees), and arachnids (e.g. spiders) are fossorial.

A spermatophore, from Ancient Greek σπέρμα (spérma), meaning "seed", and -φόρος (-phóros), meaning "bearing", or sperm ampulla is a capsule or mass containing spermatozoa created by males of various animal species, especially salamanders and arthropods, and transferred in entirety to the female's ovipore during reproduction. Spermatophores may additionally contain nourishment for the female, in which case it is called a nuptial gift, as in the instance of bush crickets. In the case of the toxic moth Utetheisa ornatrix, the spermatophore includes sperm, nutrients, and pyrrolizidine alkaloids which prevent predation because it is poisonous to most organisms. However, in some species such as the Edith's checkerspot butterfly, the "gift" provides little nutrient value. The weight of the spermatophore transferred at mating has little effect on female reproductive output.

The tiger salamander (Ambystoma tigrinum) is a species of mole salamander and one of the largest terrestrial salamanders in North America.