U.S. Route 29 (US 29) is a major north–south route in the commonwealth of Virginia. It covers 248.0 miles (399.1 km) from the North Carolina border at the city of Danville to the Key Bridge in Washington DC. US 29 roughly bisects Virginia into eastern and western halves and, along with Interstate 81 (I-81) and US 11 in western Virginia and I-85/I-95 as well as US 1 farther east, provides one of the major north–south routes through the commonwealth.
Since 1928, when the Virginia General Assembly passed Senate Bill 64, much of US 29 in Virginia is known as the Seminole Trail. Through Northern Virginia, it is known as the Lee Highway, except in Falls Church, where it acts as the east–west divider for city streets and is called North or South Washington Street, Arlington, where it was renamed Langston Boulevard in July 2021 in honor of John Mercer Langston, and Fairfax County, where it was renamed to Route 29 in 2023. On April 7, 1993, the Virginia General Assembly officially designated the entire length of US 29 from the North Carolina border to the Potomac River as the "29th Infantry Division Memorial Highway" in honor of the 29th Infantry Division, which, along with the 1st Infantry Division, formed the spearhead of the U.S. infantry that landed on the morning of June 6, 1944, on Omaha Beach in Normandy as part of the liberation of France during World War II. In addition, the name of this highway serves to honor many members of the Virginia Army National Guard who serve as part of this National Guard Division today. Signs indicating this designation have been placed periodically on both sides of US 29.