Hernán Pérez de Quesada in the context of Tundama

New Christian (Latin: Novus Christianus; Spanish: Cristiano Nuevo; Portuguese: Cristão-Novo; Catalan: Cristià Nou; Ladino: Kristiano Muevo; Arabic: المسيحيون الجدد) was a socio-religious designation and legal distinction referring to the population of former Jewish and Muslim converts to Christianity in the Spanish and Portuguese empires, and their respective colonies in the New World. The term was used from the 15th century onwards primarily to describe the descendants of the Sephardic Jews and Moors that were baptized into the Catholic Church following the Alhambra Decree of 1492. The Alhambra Decree, also known as the Edict of Expulsion, was an anti-Jewish law made by the Catholic Monarchs upon the Reconquista of the Iberian Peninsula. It required Jews to convert to Roman Catholicism or be expelled from Spain. Most of the history of the "New Christians" refers to the Jewish converts, who were generally known as Conversos (or in a more derogatory fashion Marranos), while the Muslim converts were called Moriscos.

Because these conversions were achieved in part through coercion and also with the threat of expulsion, especially when it came to the Jews, the Catholic Inquisition and Iberian monarchs suspected a number of the "New Christians" of being crypto-Jews. Subsequently, the Spanish Inquisition and then the Portuguese Inquisition was created to enforce Catholic orthodoxy and to investigate allegations of heresy. This became a political issue in the kingdoms of the Portuguese–Spanish Union itself and their respective empires abroad, particularly in Spanish America, Portuguese America, and the Caribbean. Sometimes "New Christians" travelled to territories controlled by Protestant enemies of Spain, such as the Dutch Empire, the early English Empire, or Huguenot-influenced areas of the Kingdom of France such as Bordeaux, and openly practiced Judaism, which furthered suspicion of Jewish crypsis. Nevertheless, a significant number of those "New Christians" of converso ancestry were deemed by Spanish society as sincerely Catholic and they still managed to attain prominence, whether religious (St. John of the Cross, St. Teresa of Ávila, St. John of Ávila, St. Joseph of Anchieta, Tomás Luis de Victoria, Tomás de Torquemada, Diego Laynez, Francisco de Vitoria, Francisco Suárez, and others) or political (Juan de Oñate, Luis de Carvajal y de la Cueva, Hernán Pérez de Quesada, Luis de Santángel, and others).

Tisquesusa, also spelled Thisquesuza, Thysquesuca or Thisquesusha, referred to in the earliest sources as Bogotá, the Elder, (died Facatativá, 1537) was the fourth and last independent ruler (psihipqua) of Muyquytá, main settlement of the southern Muisca between 1514 and his death in 1537. The Spanish pronunciation of his name brought about the Colombian capital Bogotá. Tisquesusa was the ruler of the southern Muisca Confederation at the time of the Spanish conquest of the Muisca, when the troops led by Gonzalo Jiménez de Quesada and his brother entered the central Andean highlands.

This is a list of conquistadors who were active in the conquest of terrains that presently belong to Colombia. The nationalities listed refer to the state the conquistador was born into. Granada and Castile are currently part of Spain, but were separate states at the time of birth of the early conquistadors.

Important conquistadors and explorers were Alonso de Ojeda, who landed first at Colombian soil and founded the first settlement Santa Cruz, Rodrigo de Bastidas, who founded the oldest still remaining city Santa Marta, Pedro de Heredia, who founded the important city of Cartagena in 1533, Gonzalo Jiménez de Quesada, who was the leader of the first and main expedition into the Andes (1536–1538), with his brother second in command and many other conquistadors, 80% of whom who didn't survive, and Nikolaus Federmann and Sebastián de Belalcázar who entered the Colombian interior from the northwest and south respectively.