Costanzo Varolio, Latinized as Constantius Varolius (1543–1575) was an Italian anatomist and a papal physician to Gregory XIII.
Varolio was born in Bologna. He was a pupil of the anatomist Giulio Cesare Aranzio, himself a pupil of Vesalius. He received his doctorate in medicine in 1567. In 1569 the Senate of the University of Bologna created an extraordinary chair in surgery for him with responsibility to teach anatomy as well and where a statue of him is housed at the Anatomical Theatre of the Archiginnasio. Later he is believed to have taught at the Sapienza University of Rome although he is not listed on the roll there. Nevertheless, he is known to have had considerable success in Rome both as a physician and as a surgeon and his memorial plaque in that city refers to his great skill in removing stones. He putatively was a physician to Pope Gregory XIII, and died in Rome, where he was buried in San Marcello al Corso.
