C4 carbon fixation or the Hatch–Slack pathway is one of three known photosynthetic processes of carbon fixation in plants. It owes the names to the 1960s discovery by Marshall Davidson Hatch and Charles Roger Slack.
C4 fixation is an addition to the ancestral and more common C3 carbon fixation. The main carboxylating enzyme in C3 photosynthesis is called RuBisCO, which catalyses two distinct reactions using either CO2 (carboxylation) or oxygen (oxygenation) as a substrate. RuBisCO oxygenation gives rise to phosphoglycolate, which is toxic and requires the expenditure of energy to recycle through photorespiration. C4 photosynthesis reduces photorespiration by concentrating CO2 around RuBisCO.
